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Другие общетехнические дисциплины          Автор Andre D.          Издательство Книга по Требованию

Perturbed or not perturbed?. Structure and dynamics of fluorophore-labelled dsDNA helices probed by NMR-spectroscopy
308 стр. мягкая обложка

автор:Andre D.
издательство: Книга по Требованию
цена:3985 руб
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Improved protocols for NMR data analysis and subsequent calculations are described. Two fluorophores are examined in detail: 2-Aminopurine (2AP) and 2-Hydroxy-7-nitrofluorene(HNF) which function as base and base pair surrogates, respectively. Advances at the experimental as well as data analysis levels are shown to allow for determination of base pair lifetimes 1 ms. Thus it has been possible to prove an extended, distributed effect of 2AP incorporation on the dynamics of at least three adjacent base pairs in either direction, in spite of the small structural influence. The HNF has been introduced as a base pair surrogate but proved to be of insufficient shape complementarity to a natural Watson-Crick-type base pair. Thus significant structural as well as dynamic perturbation around the incorporation site are observed. In this book structural and dynamic perturbations in DNA helices upon incorporation of fluorophores are characterized by NMR spectroscopy. State-of-the-art NMR experiments such as measurement of Residual Dipolar Couplings, are demonstrated to enable detection of hitherto unknown, very slight structural perturbations of a DNA helix around the 2AP modification site.

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The Nunnery Tales

I began by fingering the puckered neighboring orifice and obtained a much greater degree of liveliness and elasticity from her wriggling bottom than I otherwise should have enjoyed. A pretense of piety and a sanctimonious demeanor do not always mean a girl is therefore a virgin; indeed very often when a young lady has allowed her passions to get the better of her, and has parted with her treasure to a forward lover, or has lost it by violence, she pretends to be a prude and puritan. However, this was obviously not the case with Agnes. I felt my probing cock-head encounter her obstruction, but it was hardly a barrier to my ravaging thruster. I reared back slightly at the hips and pushed forward slowly, not wishing to cause Agnes undue pain. As she didn't seem to experience any discomfort, I repeated the motion more forcefully, enjoying the sensation elicited by the passage of my shaft in her smooth canal. I found my way into Agnes' body after the second or third thrust, and that without eliciting a scream. To my delight, I perceived that whereas dear little Louise felt too much pain to appreciate my thrusts and raptures, or to reciprocate the creamy flood that I injected into her, my present victim, on the contrary, was disposed to do both, as I soon perceived from her gaspings, kissings, and wrigglings ...

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