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Другие общетехнические дисциплины          Автор Samanthi W.          Издательство Книга по Требованию

IDENTIFICATION OF PROTEINS IN BURKHOLDERIA SPECIES AND IN TICK NYMPHS. Proteomic analysis of Burkholderia species and in the blood meals of ticks
292 стр. мягкая обложка
978-3-6391-8132-6

автор:Samanthi W.
издательство: Книга по Требованию
год:2009
цена:1995 руб
- нет в наличии

Additional studies have examined the fatty acid profiles of rabbit and sheep blood fed tick nymphs using SPALDI mass spectrometry. Methods were developed for the proteomic analysis of Burkholderia species using B. vietnamiensis G4, an opportunistic pathogen as the model organism. Proteins identified from pathogenic B. pseudomallei were compared with the non- pathogenic B. thailandensis and opportunistic pathogen B. vietnamiensis proteins. Ticks are vectors that can transmit disease causing pathogens one host to another. A mass spectrometry based proteomic approach has been applied in order to identify the proteins that are responsible for pathogenicity of Burkholderia pseudomallei. Mass spectrometry based proteomics approaches were used for the analysis of proteins extracted from 4 different cellular fractions of these bacteria. Application of mass spectrometric methods to identify the host blood components from tick vectors was investigated using tick nymphs. Using mass spectrometry based proteomics; host specific proteins like hemoglobin and immunoglobulin were identified from a single tick nymph analysis.

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