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Общая биология          Издательство Книга по Требованию

Heterologous Expression of Wheat Proteins in Yeast. Optimization of the Heterologous Expression and Purification of Wheat Storage Proteins in the Yeast Saccharomyces cerevisiae
140 стр. мягкая обложка
978-3-6390-4040-1

издательство: Книга по Требованию
год:2008
цена:2414 руб
- нет в наличии

Not only for commercial or medical purposes, but also for the characterization of foreign proteins the heterologous expression plays a significant role. How can a foreign protein be expressed in a pro- or eukaryotic host? The expression of proteins from different host organisms is of big interest in the modern biotechnology. The optimization of fermentation parameter for higher biomass and protein yield are discussed. The heterologous expression of the putatively celiac disease triggering proteins of the wheat endosperm is the central point of this book. The high cell density fermentation and the applicability of different plasmid systems are discussed in the introduction. Furthermore, the adaptation of the classical protein purification to the changed host system as well as the establishment of an aqueous two phase system as alternativ protein purification are discussed. Which host is suitable for which kind of protein? How can the specific protein yield be increased by varying process parameter? The author Dr. Falk Matthaeus gives introductorily an overview of different host systems for the heterologous expression of proteins.

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