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Общая биология          Издательство Книга по Требованию

Heterologous Expression of Wheat Proteins in Yeast. Optimization of the Heterologous Expression and Purification of Wheat Storage Proteins in the Yeast Saccharomyces cerevisiae
140 стр. мягкая обложка

издательство: Книга по Требованию
цена:2414 руб
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Not only for commercial or medical purposes, but also for the characterization of foreign proteins the heterologous expression plays a significant role. How can a foreign protein be expressed in a pro- or eukaryotic host? The expression of proteins from different host organisms is of big interest in the modern biotechnology. The optimization of fermentation parameter for higher biomass and protein yield are discussed. The heterologous expression of the putatively celiac disease triggering proteins of the wheat endosperm is the central point of this book. The high cell density fermentation and the applicability of different plasmid systems are discussed in the introduction. Furthermore, the adaptation of the classical protein purification to the changed host system as well as the establishment of an aqueous two phase system as alternativ protein purification are discussed. Which host is suitable for which kind of protein? How can the specific protein yield be increased by varying process parameter? The author Dr. Falk Matthaeus gives introductorily an overview of different host systems for the heterologous expression of proteins.

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The Gates of November

Each of the children had a private bedroom, and their two nannies slept in a room nearby. The staff, all Chinese, consisted of a cook, a maid, Solomons secretary-translator, whose Russian was perfect, and a courier. The courier rode a bicycle to deliver press releases from Solomon to Chinese and foreign press bureaus and to pick up newspapers and releases for Solomon. A stairway led from the rear hallway off the living room down to the basement, where there were storage rooms, the kitchen, and a room that contained a rotary press. The apparent serenity inside the Soviet compound was a false mirror of events outside. China was experiencing its own savage civil war. Sun Yatsen had died in 1925, his dream of a united and Westernized China unfulfilled. Chiang Kai-shek, appointed by Sun to head the new Whampoa Military Academy set up to train the officer corps of the Kuomintang army, was now commander in chief of a military force that had begun to move north from Canton, subduing the countryside and predatory private armies ...

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